LS1 - Live cell imaging, and intracellular dynamics
Nowadays, a number of technologies make it possible to analyze biological processes directly in living organisms and cells, with the ultimate goal to localize and describe in vivo the dynamics of cell metabolic pathways. Live cell imaging allows following cell populations, individual cells or specific molecules within complex living tissues and organs, while light and electron microscopy offer the possibility to assemble snapshots of events to obtain the dynamic pattern. This symposium will focus on the visualization and analysis of dynamic cell processes using various microscopy techniques, as well as on using experimental tools (e.g., optogenetics and novel probes) for monitoring cellular and tissue events.
– Manuela Malatesta, Verona University, Italy
– Pavel Hozak, Institute of Molecular Genetics CAS, Prague, Czech Republic
– Prof. Marco Biggiogera, Department of Biology and Biotechnology, University of Pavia, Pavia, Italy
– Dr. Joel Ryan, Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), LMU Munich, Germany
"Single-molecule tracking reveals distinct behaviors of epigenetic modifiers Tet1 and Tet2 in live embryonic stem cells"
LS2 - Structure and imaging of biomolecules
Imaging of cellular and subcellular structures at the microscopic level is essential for the understanding important biological processes. Advanced microscopy techniques such as conventional confocal, lightsheet, multiphoton and super-resolution (STED) microscopes allow visualization of the dynamic processes on a time-based manner. The use of fluorescence is advantageous in labeling the multiple structures and thus permits visualization of the interactions between cellular structures. Furthermore, the structure of individual biomolecules can be addressed by cryo-electron microscopy (cryo-EM) techniques. In this session, the advantages and disadvantages of using advanced microscopy techniques for detecting biomolecules and determining their structure will be discussed.
– Sevinc Inan, Dep. of Histology and Embryology, Izmir University of Economics, Izmir, Turkey
– Tea Pavkov-Keller, Institute of Molecular Biosciences (IMB), University of Graz, Graz, Austria
– Prof. Sercin Karahuseyinoglu, MD, Koç University School of Medicine, Department of Histology & Embryology, Istanbul, Turkey; Co-director, Cellular and Molecular Imaging Center, Koç University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey.
"The advantages and limitations of advanced imaging for biological structures in live and fixed cells"
– Dr. Lavinia Gambelli, University of Exeter, Living Systems Institute, Exeter, UK
"Architecture and modular assembly of the Sulfolobus S-layer revealed by electron cryo-tomography"
LS3 - Microscopic applications in symbiotic interactions, plants, microorganisms, and environmental sciences
Microscopic techniques have wide application in biological and environmental sciences. Light microscopy has recently experienced an incredible increase in technology and methods development, enabling use in study cellular features and architectures, molecular movement and protein localization, as well as morphology of microscopic specimens and samples. Electron microscopy techniques have revolutionized studies of cellular ultrastructures and organelles, with a special contribution of ESEM which is designed for imaging specimens in their natural state. Modern environmental studies utilize microscopy to study symbiotic interactions, biofilms, and anthropogenic interventions and their impacts on the environment. This session is aimed to present novel achievements in microscopic applications in botany, microbiology and environmental sciences.
– Hrvoje Fulgosi, Division of Molecular Biology, Ruđer Bošković Institute, Zagreb, Croatia
– Sonja Duletić Laušević, Faculty of Biology, University of Belgrade, Belgrade, Serbia
– Dario Hruševar, Division of Botany, Department of Biology, Faculty of Science, University of Zagreb, Croatia
"Multiproxy study of environmental changes during the last 10 000 years - example from Blatuša mire, central Croatia"
– Željko Savković, Institute of Botany and Botanical Garden „Jevremovac“, Faculty of Biology, University of Belgrade, Belgrade, Serbia
"Microscopic techniques as an expedient tool for binding science and art"
LS4 - Neuroscience and histopathology
Neuroscience and neuroscientists are among the first beneficiaries of the amazing development of imaging techniques in both light and electron microscopy. Super-resolution techniques have reached <20 nm resolution, due to fast imaging systems we can follow intercellular processes in situ, and the number of publications involving the use of the Nobel-prize awarded ultra-cryo electron microscopy is rapidly increasing. This session wants to offer a range of lectures including topics of histopathology, in which classical and state-of-the art microscopic techniques contributed to significant discoveries in the field of neuroscience.
– Agnes Kittel, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
– Gerd Leitinger, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria
– Dr. Benjamin Gottschalk, Division of Molecular Biology and Biochemistry, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging Medical University of Graz, Austria
– Dr. Ádám Dénes, Laboratory of Neuroimmunology, Dep. Molecular and Developmental Neuroscience, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
LS5 - High-resolution microscopy in life sciences
Fluorescent microscopy is a well-established method for the non-invasive measurements of cell structures and processes; however, its resolution is limited. Popularization of super-resolution imaging techniques, with superior resolution, has allowed us to probe in detail cell structures that were previously only in domain of electron microscopy. In the field of electron microscopy, advancements in detectors, image processing and reconstruction software make possible currently to study larger biological structures at near atomic-resolution, understand their molecular dynamics and functions. Nonetheless, different types of microscopies are complementary and together can lead to new biological insights. The focus of this session will be to present recent discoveries in cell/tissue structure and function using advanced microscopy techniques.
– Jernej Jorgačevski, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia
– Marie Vancová, Biology Centre CAS, Ceské Budějovice, Czech Republic
– Dr. Nataša Resnik, University of Ljubljana, Ljubljana, Slovenia
LS6 - Nanomaterials in biology and medicine
The use of nanobiotechnology in human health has been increased in recent years. Drug carrier nanoparticles with their wide range of uses and advantages are promising approaches for the treatment of many diseases. Engineered nanomaterials are made to be attracted only by diseased cells and not by normal cells. These materials allow early detection and treatment of many diseases. One of the most challenging tasks in the fields of microscopic sciences is to visualize and identify the complex interaction of nanomaterials with biological material and correlate them with specific cellular functions in physiology and pathology. The topics of the session are: carbon-based, metal-based nanomaterials and nanoparticles such as, chitosan, alginate, polymeric micelles, cellulose, liposomes, dendrimers, inorganic nanoparticles, nanocrystals, metallic nanoparticles, quantum dots, protein and polysaccharides. The evaluation of cell death in the nanosystems as disease therapy; nanotoxicology mechanisms evaluations; advantages of nanoparticles and their role in oxidative stress are important topics to be addressed in this session.
– Serap Arbak, Dep. of Histology and Embriology, Acibadem Mehmet Ali Aydinlar University, Turkey
– Stefania Meschini, ISS, Roma, Italy
– Prof. Manuela Malatesta, Department of Neuroscience, Biomedicine and Movement, Section of Anatomy and Histology, Verona University, Verona, Italy
– Prof. Feray Bakan, Nanotechnology Research and Application Center, Sabanci University, Gebze, Istanbul, Turkey
LS7 - Multidisciplinary approaches for medical and biological sciences
In biological and medical research, multidisciplinary approaches have become of great importance and interest for the scientific community. This session is focused on multiple applications and translational research in this field. Oral and poster presentations from biotechnology, biomedicine, diagnostics, and related multidisciplinary studies, are cordially invited. Researches obtained by using microscopical and imaging techniques, as well as technological innovations, are particularly welcome.
– Elisabetta Falcieri, Urbino University, Italy
– Melek Ozturk, Dep. of Medical Biology, Istanbul University, Istanbul, Turkey
– Assist. Prof. Elif Nur Firat-Karalar, Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey
"Dissecting the function of centriolar satellites as key regulators of mammalian centrosomes and cilia"
– Dr. Stefania Meschini, Istituto Superiore di Sanità, Rome, Italy
"Drug resistance in cancer and its overcome"
LS8 - Emerging and miscellaneous topics in life sciences
Microscopic methods are rapidly advancing, offering new technologies to address novel problems in biological and biomedical research. This panel is initially intended for emerging topics that may not match directly to other sessions. The session will remain open for late breaking submissions until the end of August 2019. However, the latter submissions will be assigned as poster presentations.
– Jana Nebesářová, Biology Centre CAS, Ceské Budějovice, Czech Republic
– Nela Puškaš, School of Medicine, University of Belgrade, Belgrade, Serbia
– Prof. Vladana Vukojević, Karolinska Institute, Department of Clinical Neuroscience, Center for Molecular Medicine, Stockholm, Sweden
– Prof. Jozef Kaiser, Brno University of Technology, CEITEC - Central European Institute of Technology, Brno, Czech Republic